| Local Health Department | Phone Number | Counties Served |
| Central District Health Department | 308-385-5175 | Merrick, Hall, Hamilton |
| Dakota County Health Department | 402-987-2164 | Dakota |
| Douglas County Health Department | 402-444-7471 | Douglas |
| East Central District Health Department | 402-563-9656 | Boone, Nance, Platte, Colfax |
| Elkhorn Logan Valley Public Health Department | 402-529-2233 | Madison, Stanton, Cuming, Burt |
| Four Corners Health Department | 402-362-2621 | Polk, Butler, York, Seward |
| Lincoln Lancaster County Health Department | 402-441-8000 | Lancaster |
| Loup Basin Public Health Department | 308-346-5795 | Blaine, Loup, Garfield, Wheeler, Custer, Valley, Greeley, Sherman, Howard |
| North Central District Health Department | 402-336-2406 | Cherry, Keya Paha, Boyd, Brown, Rock, Holt, Knox, Antelope, Pierce |
| Northeast Nebraska Public Health Department | 402-375-2200 | Cedar, Dixon, Wayne, Thurston |
| Panhandle Public Health District | 308-633-2866 | Sioux, Dawes, Box Butte, Sheridan, Scotts Bluff, Morrill, Garden, Grant, Banner, Kimball, Cheyenne, Deuel |
| Public Health Solutions District Health Department | 402-826-3880 | Filmore, Saline, Thayer, Jefferson, Gage |
| Sarpy/Cass Health Department | 402-339-4334 | Sarpy, Cass |
| South Heartland District Health Department | 402-462-6211 | Adams, Clay, Webster, Nuckolls |
| Southeast District Health Department | 402-274-3993 | Otoe, Johnson, Nemaha, Pawnee, Richardson |
| Southwest Nebraska Public Health Department | 308-345-4223 | Keith, Perkins, Chase, Hayes, Frontier, Dundy, Hitchcock, Red Willow, Furnas |
| Three Rivers Public Health Department | 402-727-5396 | Dodge, Washington, Saunders |
| Two Rivers Public Health Department | 888-669-7154 | Dawson, Buffalo, Gosper, Phelps, Kearney, Harlan, Franklin |
| West Central District Health Department | 308-696-1201 | Hooker, Thomas, Arthur, McPherson, Logan, Lincoln |